| 2001 | Gabriela GONZÁLEZ (MEX) | Selección México |
|---|---|---|
| 2002 | Gabriela GONZÁLEZ (MEX) | Selección México |
| 2003 | Evelyn GARCIA (ESA) | El Salvador |
| 2004 | Cindy MORALES (GUA) | ADD Jalapa |
| 2005 | María Dolores MOLINA (GUA) | Combinado SAC. GUA. |
| 2006 | María Dolores MOLINA (GUA) | Toyota Guatemala |
| 2007 | Adriana ROJAS (CRC) | Costa Rica - Toyota |
| 2008 | Adriana TOVAR (COL) | Futura Colombia |
| 2009 | Evelyn GARCIA (ESA) | El Salvador |
| 2010 | Evelyn GARCIA (ESA) | El Salvador |
| 2011 | Verónica LEAL (MEX) | Selección México |
| 2012 | Cynthia LEE (GUA) | ADD San Marcos |
| 2013 | Cynthia LEE (GUA) | ADD San Marcos |
| 2014 | Emelyn GALICIA (GUA) | ADD Quetzaltenango |
| 2015 | Flory DE LEÓN (GUA) | Hino - Pizza Hut |
| 2016 | Cynthia LEE (GUA) | ADD San Marcos |
| 2017 | Gabriela SOTO (GUA) | ADD Quetzaltenango |
| 2018 | Marcela PRIETO (MEX) | Swapit Agolico |
| 2019 | Blanca Liliana MORENO (COL) | Astana Womens Team |
| 2020 | non disputata | |
Vuelta International Femenina a Guatemala
| 2021 | Yeny Lorena COLMENARES (COL) | Colombia Tierra de Atletas - GW Bicicletas |
|---|---|---|
| 2022 | cancellata | |
Vuelta Ciclistica International Femenina a Guatemala
| 2023 | Lilibeth CHACÓN (COL) | Clarus Merquimia Group - Strongman |
|---|---|---|
| 2024 | Valentina BASILICO (ITA) | Eneicat - CMTeam |
| 2025 | Jessica Marcela PARRA (COL) | Pato Bike BMC Team |