| 2001 | Marisol PONCIANO (MEX) | Selección México |
|---|---|---|
| 2002 | Gabriela CASTAÑEDA (GUA) | Pollo Rey Guatemala |
| 2003 | Gabriela CASTAÑEDA (GUA) | Pollo Rey |
| 2004 | Sonia Briseida HERNÁNDEZ (ESA) | Banco Cuscatlán ESA |
| 2005 | Gabriela LÓPEZ (GUA) | ADD San Marcos |
| 2006 | Paola MAZARIEGOS (GUA) | ADD Jalapa |
| 2007 | Marcela RUBIANO (CRC) | Costa Rica - Toyota |
| 2008 | Sandra VALENCIA (COL) | Futura Colombia |
| 2009 | Casandra RODRÍGUEZ (CRC) | Costa Rica |
| 2010 | Ana Teresa CASAS (MEX) | Selección México |
| 2011 | Ana Teresa CASAS (MEX) | Selección México |
| 2012 | Cristha GHENT (USA) | Universidad Colorado |
| 2013 | Madeleine PAPE (USA) | Iscorp (USA) |
| 2014 | Yamilet JIMÉNEZ (GUA) | ADD Quetzaltenango |
| 2015 | Emelyn GALICIA | ADD Quetzaltenango |
| 2016 | Cynthia LEE (GUA) | ADD San Marcos |
| 2017 | Gabriela SOTO (GUA) | ADD Quetzaltenango |
| 2018 | Paola Andrea MUÑOZ GRANDON (CHI) | Swapit Agolico |
| 2019 | Katherin MONTOYA (COL) | - |
| 2020 | non disputata | |
Vuelta International Femenina a Guatemala
| 2021 | Jannie Milena SALCEDO (COL) | Colombia Tierra de Atletas - GW Bicicletas |
|---|---|---|
| 2022 | cancellata | |
| 2023 | Yesi Tatiana DUEÑAS GOMEZ (COL) | Clarus Merquimia Group - Strongman |
| 2024 | Karen GONZALEZ RAMIREZ (COL) | Sistecredito - GW |
| 2025 | Karen Lorena VILLAMIZAR (COL) | Pato Bike BMC Team |